Polyclonal Antibody to Cyclin B1 (phospho-Ser147)
The protein encoded by Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34 (cdc2) to form the maturation-promoting factor (MPF) . Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.
Product Specifications
Gene Name
CCNB1
Gene ID
891
UniProt
P14635
Host
Rabbit
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of Serine 147 (A-F-S (p) -D-V) derived from Human Cyclin B1.
Target Antigen
Cyclin B1 (phospho-Ser147)
Clonality
Polyclonal
Applications
WB, IHC, IF
Format
Purified
Components
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Applications Notes
Predicted MW: 60kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Isotype
Rabbit IgG
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