Polyclonal Antibody to eIF2 Alpha (phospho-Ser49)
Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. Kimball, S.R. (1999) Int. J. Biochem. Cell Biol. 31, 25-29. De Haro, C. et al. (1996) FASEB J. 10, 1378-1387. Sheikh, M.S. and Fornace Jr., A.J. (1999) Oncogene 18, 6121-6128. Cheshire, J.L. et al. (1999) J. Biol. Chem. 274, 4801-4806.
Product Specifications
Product Name Alternative
Eukaryotic translation initiation factor 2 subunit alpha
Gene Name
EIF2S1
Gene ID
1965
UniProt
P05198
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of Serine 49 (L-L-S (p) -E-L) derived from Human eIF2a.
Target Antigen
EIF2 Alpha (phospho-Ser49)
Clonality
Polyclonal
Applications
WB, IHC, IF
Format
Purified
Components
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Applications Notes
Predicted MW: 38kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Isotype
Rabbit IgG
Frequently Asked Questions
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