Polyclonal Antibody to PKM2 (phospho-Ser37)
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. Weiwei Yang1, Yanhua Zheng1, Yan Xia1, Haitao Ji, ERK1/2-dependent phosphorylation and nucleartranslocation of PKM2 promotes the Warburg effect." Nature Cell Biology (2012) |doi:10.1038/ncb2629
Product Specifications
Product Name Alternative
PKM||PK3||OIP3||PK2
Gene Name
PKM
Gene ID
5315
UniProt
P14618
Host
Rabbit
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of serine 37 (I-D-S (p) -P-P) derived from Human PKM2.
Target Antigen
PKM2 (phospho-Ser37)
Clonality
Polyclonal
Applications
WB, IF
Format
Purified
Components
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Applications Notes
Predicted MW: 60kd, Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Isotype
Rabbit IgG
Frequently Asked Questions
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