Polyclonal Antibody to eIF2 Alpha (Phospho-Ser51)
Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. Xavier Saelens, et al. (2001) J. Biol. Chem; 276: 41620 - 41628. Hiroyuki Kubota, et al. (2003) J. Biol. Chem ; 278: 20457 - 20460. Shijian Chu, et al. (2006) Am J Physiol Lung Cell Mol Physiol ; 291: L983 - L992. Eileen Connolly, et al. (2006) Mol. Cell. Biol ; 26: 3955 - 3965.
Product Specifications
Product Name Alternative
Eukaryotic translation initiation factor 2 subunit alpha|| EIF-2A
Gene Name
EIF2S1
Gene ID
1965
UniProt
P05198
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of serine 51 (E-L-S (p) -R-R) derived from Human eIF2a.
Target Antigen
EIF2 Alpha (Phospho-Ser51)
Clonality
Polyclonal
Applications
WB, IHC, IF
Format
Purified
Components
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Applications Notes
Predicted MW: 38kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Isotype
Rabbit IgG
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