Polyclonal Antibody to HSF1 (phospho-Ser303)
DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. Parvaneh Rafiee, et, al. (2006) Am J Physiol Cell Physiol ; 291: C931 - C945 Fumika Shinozaki, et, al. (2006) J. Biol. Chem ; 281: 16361 - 16369. Eiichi Takaki, et, al. (2006) J. Biol. Chem ; 281: 4931 - 4937. Jan-Jong Hung, et, al. (1998) J. Biol. Chem ; 273: 31924.
Product Specifications
Product Name Alternative
HSTF 1
Gene Name
HSF1
Gene ID
3297
UniProt
Q00613
Host
Rabbit
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of serine 303 (P-P-S (p) -P-P) derived from Human HSF1.
Target Antigen
HSF1 (phospho-Ser303)
Clonality
Polyclonal
Applications
WB, IHC
Format
Purified
Components
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Applications Notes
Predicted MW: 82kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Isotype
Rabbit IgG
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