Polyclonal Antibody to eIF4E (Phospho-Ser209)
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Li BD, et al. (1998) Ann Surg; 227 (5) : 756-763 Altmann M, et al. (1989) Nucleic Acids Res; 17 (18) : 7520 De Gregorio E, et al. (2001) RNA; 7 (1) : 106-113 Gu W, et al. (2004) Nucleic Acids Res; 32 (15) : 4448-4461
Product Specifications
Product Name Alternative
MRNA cap-binding protein|| eIF-4F 25 kDa subunit
Gene Name
EIF4E
Gene ID
1977
UniProt
P06730
Host
Rabbit
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of serine 209 (S-G-S (p) -T-T) derived from Human eIF4E.
Target Antigen
EIF4E (Phospho-Ser209)
Clonality
Polyclonal
Applications
WB, IHC, IF
Format
Purified
Components
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Applications Notes
Predicted MW: 25kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Isotype
Rabbit IgG
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