Polyclonal Antibody to 4E-BP1 (Phospho-Thr45)
4E-BP1 encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. Gingras AC, et al. (1998) Genes Dev 12 (4) : 502-513. Brugarolas J, et al. (2004) Genes Dev 18 (23) : 2893-2904. Kumar V, et al. (2000) EMBO J 19 (5) : 1087-1097. Moody CA, et al. (2005) J Virol 79 (9) : 5499-5506. Burnett PE, et al. (1998) Proc Natl Acad Sci U S A 95 (4) : 1432-1437.
Product Specifications
Product Name Alternative
EIF4EBP1|| PHAS-1
Gene Name
EIF4EBP1
Gene ID
1978
UniProt
Q13541
Host
Rabbit
Reactivity
Rat, Mouse, Human
Immunogen
Peptide sequence around phosphorylation site of threonine 45 (S-T-T (p) -P-G) derived from Human 4E-BP1.
Target Antigen
4E-BP1 (Phospho-Thr45)
Clonality
Polyclonal
Applications
IHC, WB
Format
Purified
Components
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Applications Notes
Predicted MW: 18kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Isotype
Rabbit IgG
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