Polyclonal Antibody to IkB-epsilon (Phospho-Ser22)
Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes. Shirane M, et al. (1999) J Biol Chem; 274 (40) : 28169-74 Karin, M. and Ben-Neriah, Y. (2000) Annu. Rev. Immunol. ;18, 621-663. Chen, Z.J. et al. (1996) Cell; 84, 853-862. Brown, K. et al. (1995) Science; 267, 1485-1488.
Product Specifications
Product Name Alternative
I-kappa-B-epsilon, IKBE, IkappaBepsilon, NF-kappa-BIE, NF-kappaB inhibitor epsilon
Gene Name
NF-KBie
Gene ID
18037
UniProt
O54910
Host
Rabbit
Reactivity
Mouse, Human
Immunogen
Peptide sequence around phosphorylation site of serine 22 (I-E-S (p) -L-R) derived from Mouse IkB-e.
Target Antigen
IkB-epsilon (Phospho-Ser22)
Clonality
Polyclonal
Applications
IHC, WB
Format
Purified
Components
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Applications Notes
Predicted MW: 40kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Isotype
Rabbit IgG
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