Polyclonal Antibody to Paxillin (Phospho-Tyr31)
An antiphosphotyrosine antibody was used to identify proteins that are phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts, and a 76-kD protein was obtained that localizes to focal adhesions at the ends of actin-containing stress fibers in nontransformed cells (Ref.1) . This protein was purified from chicken gizzard smooth muscle, and was named Pxn (Paxillin) ('paxillus' means 'small stake' or 'peg' in Latin) as a protein tethered to the membrane at focal adhesions Davidson D, et al. (2001) EMBO J 20 (13) : 3414-3426. Fleming I, et al. (1999) Proc Natl Acad Sci U S A 96 (3) : 1123-1128. Goldberg MB. (2001) Microbiol Mol Biol Rev 65 (4) : 595-626. Kook S, et al. (2000) Mol Biol Cell 11 (3) : 929-939.
Product Specifications
Product Name Alternative
Paxillin; Pxn
Gene Name
PXN
Gene ID
5829
UniProt
P49023
Host
Rabbit
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of tyrosine 31 (T-P-Y (p) -S-Y) derived from Human Paxillin.
Target Antigen
Paxillin (Phospho-Tyr31)
Clonality
Polyclonal
Applications
WB
Format
Purified
Components
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Applications Notes
Predicted MW: 68kd, Western blotting: 1:500~1:1000
Isotype
Rabbit IgG
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