Polyclonal Antibody to Paxillin (Phospho-Tyr31)

An antiphosphotyrosine antibody was used to identify proteins that are phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts, and a 76-kD protein was obtained that localizes to focal adhesions at the ends of actin-containing stress fibers in nontransformed cells (Ref.1) . This protein was purified from chicken gizzard smooth muscle, and was named Pxn (Paxillin) ('paxillus' means 'small stake' or 'peg' in Latin) as a protein tethered to the membrane at focal adhesions Davidson D, et al. (2001) EMBO J 20 (13) : 3414-3426. Fleming I, et al. (1999) Proc Natl Acad Sci U S A 96 (3) : 1123-1128. Goldberg MB. (2001) Microbiol Mol Biol Rev 65 (4) : 595-626. Kook S, et al. (2000) Mol Biol Cell 11 (3) : 929-939.