Polyclonal Antibody to PAK1/PAK2/PAK3 (Phospho-Thr423/Thr402/Thr421)
The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. Parsons M, et al. (2005) Mol Cell Biol ; 25:1680-1695. Jung JH, et al. (2005) J Biol Chem; 280:40025-40031. Lampugnani MG, et al. (2002) Mol Biol Cell ; 13: 1175-1189 Wang RA, et al. (2002) EMBO J; 21: 5437 - 5447.
Product Specifications
Product Name Alternative
P21-activated kinase 1|| PAK-1|| p65-PAK|| Alpha-PAK
Gene Name
PAK1
Gene ID
5058
UniProt
Q13153
Host
Rabbit
Reactivity
Rat, Mouse, Human
Immunogen
Peptide sequence around phosphorylation site of threonine 423 /402/421 (R-S-T (p) -M-V) derived from Human PAK1/PAK2/PAK3.
Target Antigen
PAK1/PAK2/PAK3 (Phospho-Thr423/Thr402/Thr421)
Clonality
Polyclonal
Applications
IHC, IF
Format
Purified
Components
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Applications Notes
Predicted MW: 61-67 (PAK2) 68-74 (PAK1/3) kd, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Isotype
Rabbit IgG
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