Polyclonal Antibody to eNOS (Phospho-Ser1177)
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF) -induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Fulton, D. et al. (1999) Nature 399, 597-601. Harris, M.B. et al. (2001) J. Biol. Chem. 276, 16587-16591. Thomas, S.R. et al. (2002) J. Biol. Chem. 277, 6017-6024.
Product Specifications
Product Name Alternative
Constitutive NOS|| EC-NOS|| ECNOS|| NOS3|| NOSIII
Gene Name
NOS3
Gene ID
4846
UniProt
P29474
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of serine 1177 (T-Q-S (p) -F-S) derived from Human eNOS.
Target Antigen
ENOS (Phospho-Ser1177)
Clonality
Polyclonal
Applications
WB
Format
Purified
Components
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Applications Notes
Predicted MW: 133kd, Western blotting: 1:500~1:1000
Isotype
Rabbit IgG
Frequently Asked Questions
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