Polyclonal Antibody to Src (Phospho-Tyr529)
Proto-oncogenic cytoplasmic tyrosine kinase of the SRC family. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Phosphorylation of an activation loop tyrosine activates the enzyme; phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme. Pyper J.M., (1985) Mol. Cell. Biol. 5:831-838 Pyper J.M. (1990) Mol. Cell. Biol. 10:2035-2040 Xu W., (1997) .Nature 385:595-602 Benes C.H., (2005) Cell 121:271-280
Product Specifications
Product Name Alternative
C-SRC|| SRC1
Gene Name
SRC
Gene ID
6714
UniProt
P12931
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of tyrosine 529 (P-Q-Y (p) -Q-P) derived from Human Src.
Target Antigen
Src (Phospho-Tyr529)
Clonality
Polyclonal
Applications
WB, IHC, IF
Format
Purified
Components
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Applications Notes
Predicted MW: 60kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Isotype
Rabbit IgG
Frequently Asked Questions
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