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Polyclonal Antibody to Src (Phospho-Tyr529)

Proto-oncogenic cytoplasmic tyrosine kinase of the SRC family. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Phosphorylation of an activation loop tyrosine activates the enzyme; phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme. Pyper J.M., (1985) Mol. Cell. Biol. 5:831-838 Pyper J.M. (1990) Mol. Cell. Biol. 10:2035-2040 Xu W., (1997) .Nature 385:595-602 Benes C.H., (2005) Cell 121:271-280

Product Specifications

Product Name Alternative

C-SRC|| SRC1

Gene Name

SRC

Gene ID

6714

UniProt

P12931

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Peptide sequence around phosphorylation site of tyrosine 529 (P-Q-Y (p) -Q-P) derived from Human Src.

Target Antigen

Src (Phospho-Tyr529)

Clonality

Polyclonal

Applications

WB, IHC, IF

Format

Purified

Components

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage Conditions

Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Predicted MW: 60kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Isotype

Rabbit IgG

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