Polyclonal Antibody to Akt (Phospho-Ser473)
General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I) . Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. Baudhuin LM, et al. (2004) FASEB J Feb; 18 (2) : 341-3. Min YH, et al. (2004) Cancer Res; 64 (15) : 5225-31. Feng J, et al. (2004) J Biol Chem; 279 (34) : 35510-7.
Product Specifications
Product Name Alternative
C-AKT|| PKB|| PKB-alpha|| RAC|| RAC-PK-alpha||akt s473||akt 473||akt ser473 antibody
Gene Name
AKT1
Gene ID
207
UniProt
P31749
Host
Rabbit
Reactivity
Rat, Mouse, Human
Immunogen
Peptide sequence around phosphorylation site of serine 473 (Q-F-S (p) -Y-S) derived from Human Akt.
Target Antigen
Akt (Phospho-Ser473)
Clonality
Polyclonal
Applications
IHC, WB
Format
Purified
Components
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Applications Notes
Predicted MW: 60kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Isotype
Rabbit IgG
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