Polyclonal Antibody to STAT1 (Phospho-Tyr701)
Signal transducer and activator of transcription that mediates signaling by interferons (IFNs) . Following type I IFN (IFN-a and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Heim M H, (1999) J Recept Signal Transduct Res. 19: 75-120. Durbin J E, et al. (1996) Cell. 84: 443-450. Meraz M A, et al. (1996) Cell. 84: 431-442. Wakao H, et al. (1994) EMBO J. 13: 2182-2191. Demoulin J, B. et al. (1999) J Biol Chem. 274: 25855-258 Ihle J N, et al. (1994) Trends Biochem Sci. 19: 222-227.
Product Specifications
Product Name Alternative
CANDF7||ISGF-3||STAT91
Gene Name
STAT1
Gene ID
6772
UniProt
P42224
Host
Rabbit
Reactivity
Mouse, Human
Immunogen
Peptide sequence around phosphorylation site of tyrosine 701 (T-G-Y (p) -I-K) derived from Human STAT1.
Target Antigen
STAT1 (Phospho-Tyr701)
Clonality
Polyclonal
Applications
IHC, WB
Format
Purified
Components
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Applications Notes
Predicted MW: 84,91kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Isotype
Rabbit IgG
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