Alexa Fluor 647 Conjugated Anti-Human C3aR Mab (Clone: HC3aRZ8)

Specificity : The mouse monoclonal antibody HC3aRZ8 recognizes an extracellular epitope of C3aR, a transmembrane chemotactic receptor for C3a anaphylatoxin. C3aR is a 7TM transmembrane protein associated with G proteins, and serves as a receptor for C3a complement fragment. It is expressed mainly on neutrophils, mast cells, basophils, eosinophils, dendritic cells, monocytes, and macrophages. Upon detection of its ligand, the activated C3aR signaling cascade results in degranulation, superoxide production, and chemotaxis.

Product Specifications

Product Name Alternative

AZ3B, HNFAG09

Gene Name

C3AR1

Gene ID

719

UniProt

Q16581

Host

Mouse

Reactivity

Human

Immunogen

Human C3aR transfectants

Target Antigen

C3aR

Clonality

Monoclonal

Clone

HC3aRZ8

Applications

FACS

Purification

Purified antibody is conjugated with Alexa Fluor® 647 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Components

Formulation : Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Applications Notes

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 Î1⁄4l reagent / 100 Î1⁄4l of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.