Alexa Fluor 488 Conjugated Anti-Human CD303 Mab (Clone:15E3)

CD303 is an approximately 38 kDa type II transmembrane glycoprotein with an extracellular C-type lectin domain. It is a specific marker of plasmacytoid dendritic cells, and plays roles in capturing of pathogen-related oligosaccharide-containing antigens by them, and in their presentation to T cells. CD303 also mediates a potent inhibition of interferon alpha/beta production in plasmacytoid dendritic cells, thus it represents a potential target for lupus erythematosus therapy. Specificity: The mouse monoclonal antibody 15E3 recognizes an extracellular epitope of human CD303, a transmembrane glycoprotein expressed on plasmacytoid dendritic cells.

Product Specifications

Product Name Alternative

BDCA2; CLEC4C; DLEC; HECL; CLECSF7; CLECSF11; PRO34150

Gene Name

CD303

Gene ID

170482

UniProt

Q8WTT0

Host

Mouse

Reactivity

Human

Immunogen

CD303 ectodomain fused with human IgG Fc domain

Target Antigen

CD303

Clonality

Monoclonal

Clone

15000

Applications

IHC-Fr, ICC, FACS

Purification

Purified antibody is conjugated with Alexa Fluor 488 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Components

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Applications Notes

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.