Alexa Fluor 488 Conjugated Anti-Human IL-2 MAb (Clone: 35C3)

IL-2 (interleukin 2) is a cytokine that is produced primarily by stimulated Th cells and its crucial role is induction of T cell proliferation. However, IL-2 also stimulates growth and differentiation of B cells, NK cells, monocytes and other cell types, such as LAK cells or oligodendrocytes and is one of the key molecules of the immune system. IL-2 signaling pathways lead to induction of Bcl-2 protein. Specificity: The mouse monoclonal antibody 35C3 recognizes human interleukin 2 (IL-2; secreted or intracellular) .

Product Specifications

Product Name Alternative

Interleukin 2

Gene Name

IL-2

Gene ID

3558

UniProt

P60568

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human IL-2

Target Antigen

IL-2

Clonality

Monoclonal

Clone

35C3

Applications

ICC, FACS, WB

Purification

Purified antibody is conjugated with Alexa Fluor 488 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography

Components

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Applications Notes

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.