PE Conjugated Anti-Human CD315 MAb (Clone: 1F11)

Specificity : The mouse monoclonal antibody 1F11 recognizes an extracellular epitope of CD315, a type I transmembrane glycoprotein expressed on keratinocytes, activated monocytes, and a subset of B cells. CD315, also known as prostaglandin F2 receptor negative regulator, is an approximately 135 kDa transmembrane glycoprotein, which associates with actin cytoskeleton, and with CD9 and CD81, but not with other tetraspanins. It seems to be involved in regulation of cell polarity and motility. CD315 is expressed mainly by keratinocytes, activated monocytes, and a subset of B cells, but it can be also used for distinguishing between strongly positive colon cancer and fibrosarcoma cells, and their negative normal cell counterparts.

Product Specifications

Product Name Alternative

Prostaglandin F2 receptor inhibitor, PF2RI, SMAP-6, PTGFRN, FPRP, EWI-F

Gene Name

PTGFRN

Gene ID

5738

UniProt

Q9P2B2

Host

Mouse

Reactivity

Human

Target Antigen

CD315

Clonality

Monoclonal

Clone

1F11

Applications

FACS

Purification

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Format

Purified

Components

Tabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage Conditions

Store at 2-8°C protected from light. Do not freeze.

Applications Notes

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.