APC Conjugated Anti-CD49f Monoclonal Antibody (Clone:GoH3)

CD49f (alpha 6 integrin) is a type I transmembrane glycoprotein, which noncovalently associates with CD29 (beta 1 integrin) to form VLA-6, and with CD104 (beta 4 integrin) to form alpha6/beta4 integrin complex. CD49f is expressed on platelets, megakaryocytes, monocytes, T cells and thymocytes. It is widely expressed on many cultured adherent cell lines and on epithelia in non-lymphoid tissues. It is important for adhesion to laminins, invasin, and merosin, hence also for cell migration, embryogenesis, metastasis, formation of hemidesmosomes in epithelia, and other processes. Specificity :The rat monoclonal antibody GoH3 recognizes an extracellular epitope of CD49f, an alpha6 integrin subunit.

Product Specifications

Product Name Alternative

Integrin subunit alpha 6 ITGA6, VLA6alpha, platelet gpIc, integrin subunit alpha 6

Gene Name

ITGA6

Gene ID

3655

UniProt

P23229

Immunogen

Mouse mammary tumor cells

Target Antigen

CD49f

Clonality

Monoclonal

Clone

GoH3

Applications

FACS

Purification

Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Format

Purified

Components

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage Conditions

Store at 2-8°C protected from light. Do not freeze.

Applications Notes

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.