APC Conjugated Anti-Hu NKp80 (Clone: 5D12)

NKp80, also known as CLEC5C or KLRF1, is a type II transmembrane glycoprotein of the C lectin family, which is expressed in 80 kDa homodimers on NK cells, and subsets of CD8+ alpha/beta T cells, and gamma/delta T cells. It belongs to the activating coreceptors, which induce cytotoxicity, and production of pro-inflammatory cytokines. Its ligand AICL is expressed on myeloid cells. Specificity :The mouse monoclonal antibody 5D12 recognizes an extracellular epitope of human NKp80 (CLEC5C), a C-type lectin family member, expressed on NK cells and subsets of T cells.

Product Specifications

Product Name Alternative

Killer cell lectin like receptor F1 KLRF1, CLEC5C

Gene Name

KLRF1

Gene ID

51348

UniProt

Q9NZS2

Host

Mouse

Reactivity

Monkey, Human

Immunogen

Recombinant human NKp80 extracellular domain

Target Antigen

NKp80

Clonality

Monoclonal

Clone

5D12

Applications

FACS

Purification

Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Components

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage Conditions

Store at 2-8°C. Do not freeze. Protect from prolonged exposure to light.

Applications Notes

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.