PE Conjugated Anti-Human CD49e (Clone: SAM1)

CD49e (VLA5 alpha) is a type I transmembrane glycoprotein of the integrin alpha subclass (intergrin 5 alpha), expressed on thymocytes, early and activated B cells, monocytes, NK cells, dendritic cells, osteoblast and endothelial cells. It binds to RGD sequence in fibronectin and to neural adhesion molecule L1. CD49e interactions are important for maintaining the integrity of the endothelial monolayer, as well as it is involved in monocyte migration, T cell costimulation, regulation of cell survival, and other. Specificity: The mouse monoclonal antibody SAM1 recognizes an extracellular epitope of CD49e (integrin 5 alpha), a transmembrane glycoprotein expressed on thymocytes, early and activated B cells, monocytes, NK cells, dendritic cells, osteoblast and endothelial cells.

Product Specifications

Product Name Alternative

VLA5 alpha; integrin 5 alpha; FNRA

Gene Name

CD49e

Gene ID

3678

UniProt

P08648

Host

Mouse

Reactivity

Human, Non-Human Primates

Immunogen

U937 cells

Target Antigen

CD49e

Clonality

Monoclonal

Clone

SAM1

Applications

FACS

Purification

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Components

Formulation:Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Applications Notes

Application details: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.