PE Congugated Anti-Human C5aR2 Antibody (Clone : 1D9-M12)

C5aR2, also known as C5L2, is one of two receptors for C5a (anaphylatoxin) . It is coexpressed with C5aR1 (CD88) in neutrophils, as well as e.g. in mast cells, astrocytes, or macrophages, and seems to have both pro-inflammatory and anti-inflammatory roles, depending on circumstances. Unlike CD88, C5aR2 is not coupled to G-protein, thus the modulatory role is more likely. Specificity : The mouse monoclonal antibody 1D9-M12 recognizes an extracellular epitope on C5aR2 (C5L2), a C5a complement receptor, which is coexpressed with C5aR1 (CD88) in neutrophils, as well as e.g. in mast cells, astrocytes, or macrophages.

Product Specifications

Product Name Alternative

C5L2, GPR77, GPF77, complement component 5a receptor 2

Gene Name

C5AR2

Gene ID

27202

UniProt

Q9P296

Host

Mouse

Reactivity

Human, Non-Human Primates

Immunogen

L1.2 cells transfected with human C5aR2

Target Antigen

C5aR2

Clonality

Monoclonal

Clone

1D9-M12

Applications

FACS

Purification

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Components

Formulation : Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Applications Notes

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.