PE Conjugated Anti-CD370 Antibody (Clone : 8F9)

CD370 / CLEC9A, also known as DNGR1, is a type II transmembrane glycoprotein with extracellular C-type lectin domain and intracellular ITAM-containing domain. Its expression is restricted to BDCA3+ conventional dendritic cells and to a subset of CD14+ CD16- monocytes. CD370 serves as a receptor for ubiquitous preformed acid-labile protein associated ligands that are exposed when the cell membrane is damaged, such as on necrotic cells. Its triggering by these ligands mediates recruitment and activation of the tyrosine kinase Syk and leads to their cross-presentation to the immune system. Specificity : The mouse monoclonal antibody 8F9 recognizes an extracellular epitope of CD370 / CLEC9A (DNGR1), a type II transmembrane protein functioning as an endocytic receptor on BDCA31+ dendritic cells and on a subset of CD14+ CD16- monocytes.

Product Specifications

Product Name Alternative

CLEC9A, DNGR1, DNGR-1, UNQ9341, C-type lectin domain containing 9A

Gene Name

CLEC9A

Gene ID

283420

Reactivity

Human

Immunogen

RBL-2H3 cells expressing human CLEC9A fused to an HA epitope

Target Antigen

CD370

Clonality

Monoclonal

Clone

8F9

Applications

FACS

Purification

The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.

Components

Formulation : Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide

Storage Conditions

Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light.

Applications Notes

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.