FITC Conjugated Anti-CD300e / IREM-2 Monoclonal Antibody (Clone: UP-H2)

CD300e / IREM-2 (immune receptor expressed by myeloid cells 2), also known as CLM2 or LMIR6, is a monomeric transmembrane glycoprotein with a single extracellular immunoglobulin-like domain. Intracellularly it associates with DAP-12, an ITAM-containing adaptor molecule. CD300e is expressed on mature monocytes and peripheral blood myeloid dendritic cells. Its crosslinking leads to release of pro-inflammatory cytokines, and increased expression of activation markers.

Product Specifications

Product Name Alternative

CD300E||CD300LE||CLM2||CMRF35A5||IREM2

Gene Name

CD300E

Gene ID

342510

UniProt

Q496F6

Host

Mouse

Reactivity

Human

Immunogen

CD300e-HA-transfected cells

Target Antigen

CD300e

Clonality

Monoclonal

Clone

UP-H2

Applications

FACS

Purification

Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Format

Purified

Components

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage Conditions

Store at 2-8°C protected from light. Do not freeze.

Applications Notes

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.