Anti-Blood Group ABH Monoclonal Antibody (Clone: HE-10) purified
The mouse monoclonal antibody HE-10 agglutinates erythrocytes of group A, and is excellent as a tumour marker in patients of blood group B and 0. It does not agglutinate erythrocytes of group B and 0. Study with specific oligosaccharides showed that the antibody HE-10 reacts with A and H antigens with chain types 3 and 4 and it does not react with A disaccharide, A trisaccharide, A type 1, A type 2, ALeb. The antibody HE-10 does not react with normal tissue sections of donors with blood group B and 0 but it reacts specifically with malignant tissues.
Product Specifications
Host
Mouse
Reactivity
Human
Immunogen
Mixture of erythrocytes of group A1 and glycoprotein fraction isolated from saliva of secretors with blood group A.
Target Antigen
Blood Group ABH
Clonality
Monoclonal
Clone
HE-10
Applications
IHC, FACS
Purification
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods) .
Format
Purified
Components
1mg/mL, Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Storage Conditions
Store at 2-8°C. Do not freeze.
Applications Notes
Flow cytometry: Recommended dilution: 1-4 μg/mL
Isotype
Mouse IgM
Conjugate
Unconjugated
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