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Anti-Blood Group ABH Monoclonal Antibody (Clone: HE-10) purified

The mouse monoclonal antibody HE-10 agglutinates erythrocytes of group A, and is excellent as a tumour marker in patients of blood group B and 0. It does not agglutinate erythrocytes of group B and 0. Study with specific oligosaccharides showed that the antibody HE-10 reacts with A and H antigens with chain types 3 and 4 and it does not react with A disaccharide, A trisaccharide, A type 1, A type 2, ALeb. The antibody HE-10 does not react with normal tissue sections of donors with blood group B and 0 but it reacts specifically with malignant tissues.

Product Specifications

Host

Mouse

Reactivity

Human

Immunogen

Mixture of erythrocytes of group A1 and glycoprotein fraction isolated from saliva of secretors with blood group A.

Target Antigen

Blood Group ABH

Clonality

Monoclonal

Clone

HE-10

Applications

IHC, FACS

Purification

Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods) .

Format

Purified

Components

1mg/mL, Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Storage Conditions

Store at 2-8°C. Do not freeze.

Applications Notes

Flow cytometry: Recommended dilution: 1-4 μg/mL

Isotype

Mouse IgM

Conjugate

Unconjugated

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