Anti-Hu CD245 PE Mab (DY12)

Specificity:The mouse monoclonal antibody DY12 recognizes an extracellular epitope of CD245, a transmembrane protein expressed mainly by peripheral blood lymphocytes, NK cells, monocytes, granulocytes and platelets. CD245 is a 220-250 kDa transmembrane protein of unknown structure. It is expressed at lower level on resting blood lymphocytes, granulocytes, platelets, and thymocytes, and at high level on monocytes and on IL-2-dependent T cell lines. It is also expressed on Ewing sarcoma and osteosarcoma cell lines. CD245 seems to act as a costimulation molecule of T and NK cells.

Product Specifications

Product Name Alternative

P220/240, DY12, DY35

Host

Mouse

Reactivity

Human

Immunogen

Human NK-type YT2C2 cells

Target Antigen

CD245

Clonality

Monoclonal

Clone

DY12

Applications

FACS

Purification

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Components

Storage Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Applications Notes

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.