Anti-Hu CD367 PE Mab (9E8)

Specificity: The mouse monoclonal antibody 9E8 recognizes an extracellular epitope of human CD367, a type II transmembrane protein of C-lectin family, expressed mainly on antigen presenting cells. CD367 is an approximately 20-28 kDa C-type lectin with immunoreceptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic part. CD367 binds in calcium-dependent manner to mannose, fucose, and weakly also to N-acetylglucosamine. It is expressed on dendritic cells, macrophages, monocytes, B cells, and neutrophils. In rheumatoid arthitis patients CD367 is expressed also on CD4+ T cells. After ligand-mediated triggering, it is internalized by clathrin-dependent endocytosis and contributes to the antigen presentation to CD8+ T cells. It may also be involved in modulation of the antigen presenting cell response.

Product Specifications

Product Name Alternative

DCIR, CLEC4A, LLIR

Gene Name

CLEC4A

Gene ID

50856

UniProt

Q9UMR7

Host

Mouse

Reactivity

Human, Non-Human Primates

Immunogen

CD367 ectodomain fused with human Fc

Target Antigen

CD367

Clonality

Monoclonal

Clone

9E8

Applications

FACS

Purification

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Components

Storage Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Applications Notes

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.