Anti-Hu CD49c APC Mab (ASC-1)

Specificity: The mouse monoclonal antibody ASC-1 recognizes an extracellular epitope of CD49c (integrin alpha 3), a transmembrane glycoprotein composed of disulfide linked 125 kDa and 30 kDa chains, and expressed on adherent cell lines and to a lesser extent on T and B cells and monocytes. CD49c / Integrin alpha 3 is a type I transmembrane glycoprotein proteolytically cleaved into two disulfide linked chains. It noncovalently associates with CD29 (integrin beta 1) to form the VLA-3 complex, an adhesion receptor for extracellular matrix components (fibronectin, laminin 1, laminin 5, entactin, and collagen) . It is expressed on adherent cells, mainly on fibroblasts, epithelial cells and endothelial cells.

Product Specifications

Product Name Alternative

ITGA3, VLA-3 alpha, FRP-2, FRP2, GAPB3

Gene Name

ITGA3

Gene ID

3675

UniProt

P26006

Host

Mouse

Reactivity

Human

Immunogen

Human SSC-9 cell line (squamous cell carcinoma)

Target Antigen

CD49c

Clonality

Monoclonal

Clone

ASC-1

Applications

FACS

Purification

Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Components

Storage Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Applications Notes

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.