Anti-Hu CD19 PE-Cy5

Specificity: The mouse monoclonal antibody 4G7 recognizes an extracellular epitope of human CD19. CD19 is a transmembrane glycoprotein of Ig superfamily expressed by B cells from the time of heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complex with CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (B cell antigen receptor), this complex signals to decrease B cell treshold for activation by the antigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signal independently of BCR coligation and it turns out to be a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.

Product Specifications

Product Name Alternative

B4, Leu-12, CVID3

Gene Name

CD19

Gene ID

930

UniProt

P15391

Host

Mouse

Reactivity

Human

Immunogen

Human CCL (chronic lymphocytic leukemia) cells

Target Antigen

CD19

Clonality

Monoclonal

Clone

4G7

Applications

FACS

Purification

Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 5 (PE-Cy5) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Components

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Applications Notes

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.