Anti-Human CD123 PerCP Mab (Clone:6H6)

Specificity : The mouse monoclonal antibody 6H6 recognizes an extracellular epitope of CD123 (interleukin 3 receptor alpha), a 60-70 kDa transmembrane protein expressed by myeloid precursors, megakaryocytes, macrophages, dendritic cells, mast cells, basophils, and some B cells. This antibody does not inhibit IL-3 binding to its receptor. CD123 is the alpha chain of interleukin 3 receptor (IL-3R alpha) . This subunit heterodimerizes with the interleukin 3 receptor beta chain (CD131), which is shared with other receptors. CD123 interacts with IL-3 specifically, but with low affinity, and association with the beta subunit confers high affinity binding to the receptor heterodimer. Both chains are required for signaling, but receptor activation and signal transduction depend on IL-3 binding to CD123 as the initial step.

Product Specifications

Product Name Alternative

IL3RA, MGC34174, hIL-3Ra

Gene Name

IL3RA

Gene ID

3563

UniProt

P26951

Host

Mouse

Reactivity

Human

Immunogen

IL3 receptor alpha chain expressed on the surface of transiently transfected COS cells

Target Antigen

CD123

Clonality

Monoclonal

Clone

6H6

Applications

FACS

Purification

Purified antibody is conjugated with activated Peridinin-Chlorophyll Protein (PerCP) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Components

Formulation : Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Applications Notes

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.