APC Conjugated Anti-Human SIGLEC10 Mab (Clone: 5G6)

Specificity : The mouse monoclonal antibody 5G6 recognizes an extracellular epitope of human SIGLEC10, a sialic acid-binding lectin expressed on subsets of human leucocytes. SIGLEC10 is a CD33-related receptor of sialoglycans, expressed on eosinophils, monocytes, a subpopulation of NK cells, and at lower level on B cells. Its murine ortholog is Siglec G. SIGLEC10 seems to act as an immunomodulatory receptor, which binds to VAP-1, a glycoprotein expressed on endothelium under inflammatory conditions. Another ligand of SIGLEC10 is CD24, a marker of poorer prognosis in carcinomas.

Product Specifications

Product Name Alternative

PRO940, SGL2, sialic acid binding Ig like lectin 10

Gene Name

SIGLEC10

Gene ID

89790

UniProt

Q96LC7

Host

Mouse

Reactivity

Human

Immunogen

SIGLEC10 extracellular domain fused with human IgG1 Fc fragment

Target Antigen

SIGLEC10

Clonality

Monoclonal

Clone

5G6

Applications

FACS

Purification

Urified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Format

Purified

Components

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage Conditions

Store at 2-8°C. Do not freeze. Avoid exposure to light.

Applications Notes

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.