Anti-Perforin FITC MAb (Clone: dG9)

Specificity: The mouse monoclonal antibody dG9 (also known as deltaG9) recognizes perforin, a 70 kDa protein expressed in cytoplasmic granules of cytotoxic T cells and NK cells. Perforin is a 70 kDa cytolytic protein with structural and functional similarities to complement component 9 (C9) . It is stored in cytoplasmic granules of cytotoxic T cells and NK cells and after its release it forms transmembrane pores in the target cells to kill it. As perforin is a key effector molecule for cell-mediated cytolysis, defects of its gene can cause severe disorders.

Product Specifications

Product Name Alternative

PRF1, P1, PFP, HPLH2, Perforin 1

Gene Name

PRF1

Gene ID

5551

UniProt

P14222

Host

Mouse

Reactivity

Human, Bovine

Immunogen

Purified granules from human YT lymphoma cell line

Target Antigen

Perforin

Clonality

Monoclonal

Clone

DG9

Applications

FACS

Purification

Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Components

Storage Buffer : Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Applications Notes

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Intracellular staining.