Anti-Hu CD52 FITC

CD52 (CAMPATH-1, HE5) is a highly glycosylated GPI-anchored 21-28 kDa glycopeptide which is present at high levels on lymphocytes, macrophages, epithelial cells of male reproductive tract and mature sperm. Its 12-amino acid beckbone carries a complex N-linked carbohydrate moiety, which differs between sperm and leukocyte CD52, as well as the GPI anchor does. CD52 can be acquired by sperm cells from seminal plasma, where it is released by epithelial cells. Although CD52 is not an essential T-cell costimulator, its triggering results in activation of normal human T cells. CD52 is a very good target for antibody/complement-mediated cell lysis.Specificity :The mouse monoclonal antibody 4C8 recognizes CD52, a GPI-anchored glycopeptide expressed on the surface of lymphocytes, macrophages, epithelial cells of male reproductive tract, and mature sperms.

Product Specifications

Product Name Alternative

CD52 molecule CAMPATH-1, HE5, ESPE5

Gene Name

CD52

Gene ID

1043

UniProt

P31358

Reactivity

Human

Immunogen

Human lymphocytes

Target Antigen

CD52

Clonality

Monoclonal

Clone

4C8

Applications

FACS

Purification

Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Components

Storage Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Applications Notes

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.