Anti-Cyclin B1 PE

Cyclin B1 is a regulatory protein involved in mitosis. It complexes with p34 (cdc2) to form maturation-promoting factor (MPF), and is necessary for proper control of the G2/M phase transition of the cell cycle. It is expressed in tissues containing proliferating cells, such as lymph node, testis et al.Specificity :The mouse monoclonal antibody V152 recognizes cyclin B1, a 48 kDa intracellular protein necessary for G2/M phase transition of the cell cycle.

Product Specifications

Product Name Alternative

Cyclin B1

Gene Name

CCNB1

Gene ID

891

UniProt

P14635

Reactivity

Mammalian

Immunogen

Recombinant His-tagged hamster cyclin B1

Target Antigen

Cyclin B1

Clonality

Monoclonal

Clone

V152

Applications

FACS

Purification

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Components

Concentration: 1 mg/mLStorage Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Applications Notes

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.