Rat IgG2a Isotype Control APC Antibody (Clone : S193)
Specificity : This rat IgG2a kappa monoclonal antibody (clone RTG2A1-1, also known as G2A-1-1 or KLH/G2A-1-1) detects specifically the hemocyanin of keyhole limpet and can be used as a negative control antibody in other species.
Product Specifications
Clonality
Monoclonal
Clone
RTG2A1-1
Applications
FACS
Purification
The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Components
0.1 mg/mLFormulation : Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Storage Conditions
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light.
Applications Notes
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/mL of incubation mixture), change the conditions of your experiment to reduce the background.
Isotype
Rat IgG2a
Conjugate
APC
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