Rat IgG2b Isotype Control Antibody (Clone : S193)

Specificity : This rat IgG2b kappa monoclonal antibody (clone RTG2B1-2, also known as G2B-1-2 or KLH/G2B-1-2) detects specifically the hemocyanin of keyhole limpet and can be used as a negative control antibody in other species.

Product Specifications

Clonality

Monoclonal

Clone

RTG2B1-2

Applications

FACS , IP, IHC (P), IHC (F), ICC, WB, ELISA

Purification

Purified by protein-A affinity chromatography

Format

Purified

Components

1 mg/mLFormulation : Phosphate buffered saline (PBS) solution with 15 mM sodium azide

Storage Conditions

Store at 2-8°C. Do not freeze.

Applications Notes

Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/mL of incubation mixture), change the conditions of your experiment to reduce the background.