Anti-Human CD41/CD61 (PAC-1 epitope) PE (Clone : PAC-1)

CD41 (platelet glycoprotein IIb, integrin alpha IIb) is composed of two subunits (120 kDa transmembrane alpha chain and 23 kDa extracellular beta chain) and interacts with CD61 (platelet glycoprotein IIIa, integrin beta 3) in the presence of calcium to form a functional adhesive protein receptor. CD41/CD61 complex is one of the earliest markers of the megakaryocytic lineage. Upon blood vessel damage, this receptor binds to a variety of proteins including von Willebrand factor, fibrinogen, fibronectin and vitronectin, and it is involved in platelet aggregation.Specificity : The mouse monoclonal antibody PAC-1 recognizes an extracellular activation-induced conformational epitope PAC-1 on CD41/CD61 complex (gpIIb/IIIa), also known as integrin alpha IIb beta 3, a receptor which mediates platelet aggregation.

Product Specifications

Product Name Alternative

Platelet GPIIb, Integrin alpha-Iib, GPalpha IIb, GPIIb, GT, GP3A, platelet GPIIIa

Gene Name

ITGA2B/ITGB3

Gene ID

3674/3690

Reactivity

Human

Immunogen

Human platelets

Target Antigen

CD41/CD61 (PAC-1 epitope)

Clonality

Monoclonal

Clone

PAC-1

Applications

FACS

Purification

The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.

Components

Formulation : Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide

Storage Conditions

Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light.

Applications Notes

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.