Anti-Human CD42b APC (Clone : AK2)

CD42b (GPIb alpha) composes together with GPIb beta, GPIX and GPV the GPIb-IX-V receptor complex critical in the process of platelet-rich thrombus formation by tethering the platelet to a thrombogenic surface. CD42b binds to von Willebrand factor (VWF) exposed at a site of vascular injury, as well as to thrombin, coagulation factors XI and XII, high molecular weight kininogen, TSP-1, integrin Mac-1 and P-selectin. The extracellular domain of CD42b by its interactions also contributes to metastasis.Specificity : The mouse monoclonal antibody AK2 recognizes an extracellular epitope of CD42b (GPIb alpha), a 135-145 kDa membrane glycoprotein expressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are composed in a disulfide linked heterodimer (CD42b/c; 160 kDa) ; CD42b/c forms a noncovalent complex with CD42a and CD42d.

Product Specifications

Product Name Alternative

BSS, GP1B, VWDP, CD42B, GPIbA, BDPLT1, BDPLT3, DBPLT3, glycoprotein Ib platelet subunit alpha

Gene Name

GP1BA

Gene ID

2811

Reactivity

Human

Immunogen

Human platelets

Target Antigen

CD42b

Clonality

Monoclonal

Clone

AK2

Applications

FACS

Purification

The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.

Components

Formulation : Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide

Storage Conditions

Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light.

Applications Notes

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.