Anti-Granzyme B FITC (Clone : CLB-GB11)

Granzyme B is a serine protease that is expressed in cytoplasmic granules of cytotoxic T lymphocytes and NK cells. Vectorial secretion of perforin and granzymes is responsible for their granule-mediated cytotoxicity. Granzyme B plays a pivotal role in the induction of apoptosis in the target cells by activation of caspases. Moreover, granzyme B was reported to cleave directly alpha-tubulin, leading to perturbation of microtubule networks during the induced cell death.Specificity : The mouse monoclonal antibody CLB-GB11 recognizes granzyme B, a 31 kDa serine protease expressed intracellularly in activated Tc cells and NK cells.

Product Specifications

Product Name Alternative

GZMB, HLP, CTLA1, SECT, granzyme B

Gene Name

GZMB

Gene ID

3002

Reactivity

Human

Immunogen

Human NK cell line YT-INDY-derived granzyme B

Target Antigen

Granzyme B

Clonality

Monoclonal

Clone

CLB-GB11

Applications

FACS

Purification

The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.

Components

Formulation : Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide

Storage Conditions

Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light.

Applications Notes

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.