Anti-CD147 / Basigin / Neurothelin Monoclonal Antibody (Clone:MEM-M6/6) -Low Endotoxin

CD147 (basigin, neurothelin, OX-47, 5A11, CE9, M6) also known as EMMPRIN (extracellular matrix metalloproteinase inducer) or TCSF (tumour cell-derived collagenase-stimulatory factor) is an ubiquitously expressed cell surface protein with multiple glycosylated forms. The highest level of CD147 expression is on metabolically active cells, such as lymphoblasts, inflammatory cells, brown adipocytes and malignant tumour cells. CD147 has multiple functions, including facilitating of cell surface expression of monocarboxylate transporter proteins and extracellular matrix metalloproteinases, regulation of integrin functions, it plays roles in cell development and activation, fetal development or retinal function.

Product Specifications

Product Name Alternative

BSG||UNQ6505/PRO21383

Gene Name

BSG

Gene ID

682

UniProt

P35613

Host

Mouse

Reactivity

Human

Immunogen

Protein A-CR purified soluble recombinant form of CD147, CD147Rg, which consists of the cDNA coding for the hinge region, CH2-and CH3 domain of human IgG1 (CD147Rg is secreted by transfectants as a dimmer) .

Target Antigen

CD147

Clonality

Monoclonal

Clone

MEM-M6/6

Applications

FACS

Purification

Purified by protein-A affinity chromatography

Format

Low Endotoxin

Components

Low Endotoxin azide free PBS. 0.2μm Filter Sterilized. 1.0 mg/mL

Storage Conditions

Store at 2-8°C. Do not freeze.

Applications Notes

Flow Cytometry Recommended dilution:4-10 μg/mL Western Blotting Positive control: 293 human fibroblastoid cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail) . Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Functional Application The antibody MEM-M6/6 (high-affinity mAb of unique epitope specificity) inhibits anti-CD3-induced T cell activation.