Anti-TNF-alpha Monoclonal Antibody (Clone:MAb11)

TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.

Product Specifications

Product Name Alternative

Tumor necrosis factor, Cachectin , Tumor necrosis factor ligand superfamily member 2

Gene Name

TNF

Gene ID

7124

UniProt

P01375

Host

Mouse

Reactivity

Human, Non-Human Primates, Pig

Immunogen

Recombinant human TNF-alpha

Target Antigen

TNF-alpha

Clonality

Monoclonal

Clone

MAb11

Applications

FACS, IHC-Fr, ICC, ELISA, Functional Assay

Purification

Purified by protein-A affinity chromatography

Format

Purified

Storage Conditions

Store at 2-8°C. Do not freeze.

Applications Notes

Flow Cytometry Application note:intracellular staining Immunohistochemistry (frozen sections) Application note:paraformaldehyde-fixed, saponin-treated frozen tissue sections Immunocytochemistry ELISA Biotinylated MAb11 can be used as a detection antibody in combination with capture antibody MAb1 Functional Application neutralization