Anti-CD326 / EpCAM Monoclonal Antibody (Clone:323/A3)

CD326 / EpCAM (also known as ESA, EGP40, EGP-2, KSA1/4, CO17-1A, GA733-2, MOC31, Ber-EP4) is a 40 kDa transmembrane glycoprotein serving as adhesion molecule in the basolateral membranes in a variety of epithelial cells. CD326 mediates calcium-independent homotypic cell-cell adhesions. CD326 over-expression has been detected in many epithelial tumours and is often associated with bad prognosis. It has been used for diagnostics of (pre-) malignancies at early stages.

Product Specifications

Product Name Alternative

EPCAM||GA733-2||M1S2||M4S1||MIC18||TACSTD1||TROP1

Gene Name

EPCAM

Gene ID

4072

UniProt

P16422

Host

Mouse

Reactivity

Human

Immunogen

Human breast cancer MCF-7 cells

Target Antigen

CD326 / EpCAM

Clonality

Monoclonal

Clone

323/A3

Applications

FACS

Purification

Purified by protein-A affinity chromatography

Format

Purified

Storage Conditions

Store at 2-8°C. Do not freeze.

Applications Notes

Western blotting: Non-reducing conditions; recommended dilution: 1-2 μg/ml; positive control: MCF-7 cells. Immunoprecipitation: Recommended dilution: 1-4 μg / ml per 100-500 μg of protein in 1 ml lysate. Immunohistochemistry (paraffin sections) : Recommended dilution: 1-10 μg/ml; pretreatment: tissue section digestion with pepsin (15 min at RT or 10 min at 37°C, 1 mg / ml Tris-HCl, pH 2.0) ; positive tissue: breast carcinoma. Immunocytochemistry: Recommended dilution: 1-10 μg/ml. Flow cytometry: Recommended dilution: 1-4 μg/mL.