Anti-CD300a Monoclonal Antibody (Clone:MEM-260)

CD300a (CMRF-35H, IRp60) is a non-MHC-specific inhibitory receptor of immunoglobulin superfamily, which contains three immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that associate with SH2-containing phosphatases SHP-1 and SHP-2. CD300a is expressed on many cell types including T cells, NK cells, neutrophils, eosinophils or mast cells. Its triggering inhibits activating signals such as those of IL5, GM-CSF or eotaxin, as well as supresses mast cell degranulation or NK cell cytotoxic activity.

Product Specifications

Product Name Alternative

CD300A||CMRF35H||IGSF12||HSPC083

Gene Name

CD300A

Gene ID

11314

UniProt

Q9UGN4

Host

Mouse

Reactivity

Human

Immunogen

HPB human acute lymphoid leukemia cell line

Target Antigen

CD300a

Clonality

Monoclonal

Clone

MEM-260

Applications

FACS

Purification

Purified by protein-A affinity chromatography

Format

Purified

Storage Conditions

Store at 2-8°C. Do not freeze.

Applications Notes

Flow Cytometry Recommended dilution:2 μg/mL Western Blotting Positive material: HPB cell line Negative material: JURKAT human cell line, RAJI human cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail) . Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer.