Anti-HLA-DR Monoclonal Antibody (Clone:MEM-12)

HLA-DR, a member of MHC class II glycoproteins, that bind intracellularly processed peptides and present them to the Th cells, is composed of 36 kDa alpha chain and 27 kDa beta chain, both anchored in the plasma membrane. Together with other MHC II molecules HLA-DR plays a central role in the immune system.

Product Specifications

Product Name Alternative

HLA class II histocompatibility antigen, DR alpha chain, MHC class II antigen DRA

Gene Name

HLA-DRA

Gene ID

3122

UniProt

P01903

Host

Mouse

Reactivity

Human

Immunogen

Thymocyte membrane

Target Antigen

HLA-DR

Clonality

Monoclonal

Clone

MEM-12

Applications

FACS

Purification

Purified by protein-A affinity chromatography

Format

Purified

Storage Conditions

Store at 2-8°C. Do not freeze.

Applications Notes

Flow Cytometry Recommended dilution:2 μg/mL Positive control: B-lymphocytes, Monocytes, DAUDI cell line, RAJI cell line Immunoprecipitation Positive control: B-lymphocytes, Monocytes, DAUDI cell line, RAJI cell line Western Blotting Positive control: B-lymphocytes, Monocytes, DAUDI cell line, RAJI cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail) . Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Do not heat/boil.