Anti-CD105 / Endoglin Monoclonal Antibody (Clone:MEM-229)

CD105 (Endoglin) is a homodimeric transmembrane glycoprotein serving in presence of TGFbetaR-2 as a receptor for TGFbeta-1 and TGFbeta-3. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumours and its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxic endothelial cells and also antagonises the inhibitory effects of TGFbeta-1 on vascular endothelial cell growth and migration. Normal cellular levels of CD105 are required for formation of new blood vessels.

Product Specifications

Product Name Alternative

ENG||END

Gene Name

ENG

Gene ID

2022

UniProt

P17813

Host

Mouse

Reactivity

Pig, Human

Immunogen

Recombinant Vaccinia virus containing the human CD105 (L-isoform) cDNA.

Target Antigen

CD105

Clonality

Monoclonal

Clone

MEM-229

Applications

ICC, IHC, FACS, WB

Purification

Purified by protein-A affinity chromatography

Format

Purified

Components

1mg/mL, Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage Conditions

Store at 2-8°C. Do not freeze.

Applications Notes

Flow cytometry: Recommended dilution: 2-6 μg/ml; positive control: Kg1 human acute myelogenous leukemia cell line. Immunohistochemistry (frozen sections) : Recommended dilution: 1:200, acetone fixation. Western blotting: Non-reducing conditions.