Anti-NTAL / LAB Monoclonal Antibody (Clone:NAP-03)

NTAL (Non-T cell Activation Linker), also known as LAB (Linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.

Product Specifications

Product Name Alternative

LAT2||LAB||NTAL||WBS15||WBSCR15||WBSCR5||HSPC046

Gene Name

LAT2

Gene ID

7462

UniProt

Q9GZY6

Host

Mouse

Reactivity

Human

Immunogen

Peptide corresponding to aa 196-212 of human NTAL coupled to KLH.

Target Antigen

NTAL / LAB

Clonality

Monoclonal

Clone

NAP-03

Applications

IP, WB, IHC

Purification

Purified by protein-A affinity chromatography

Format

Purified

Storage Conditions

Store at 2-8°C. Do not freeze.

Applications Notes

Immunoprecipitation Western Blotting Recommended dilution: 1-2 μg/mL, incubation 60 min Positive control: RAMOS human lymphoma cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail) . Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Application note: Non-Reducing conditions. 12% separating SDS-PAGE gel. Immunohistochemistry Recommended dilution: 5 μg/mL Positive tissue: tonsil, germinal center