Anti-CD38 Monoclonal Antibody (Clone:HIT2)

CD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells. Another role of CD38 is provided by enzymatic activity of its extracellular part. CD38 acts as NAD+ glycohydrolase converting NAD+ into ADP-ribose, as ADP-ribosyl cyclase producing cADPR and as cADPR hydrolase, thus affecting levels of calcium-mobilizing metabolites. ADPR produced by CD38 serves as an important second messenger of neutrophil and dendritic cell migration.

Product Specifications

Product Name Alternative

CD38

Gene Name

CD38

Gene ID

952

UniProt

P28907

Host

Mouse

Reactivity

Human

Immunogen

Human thymocytes in foetus

Target Antigen

CD38

Clonality

Monoclonal

Clone

Applications

FACS

Purification

Purified by protein-A affinity chromatography

Format

Purified

Storage Conditions

Store at 2-8°C. Do not freeze.

Applications Notes

Flow Cytometry Western Blotting Recommended dilution: 2 μg/mL Positive control: RAJI human cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail) . Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Boil for 5 min. Immunohistochemistry Recommended dilution:10 μg/mL Immunohistochemistry (frozen sections)