Anti-NHERF1 / EBP50 Monoclonal Antibody (Clone:EBP-10)

NHERF1 (Na+/H+ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50) is an adaptor protein, which associates with beta-catenin and is required for its localization at the cell-cell junctions, interacts with various G protein-coupled receptors and regulates their traffic, as well as sodium-hydrogen exchange and sodium-dependent phosphate transport. NHERF1/EBP50 inhibits cell motility and is required to suppress anchorage-independent growth. It contains C-terminal ERM (ezrin, radixin, moesin) -binding region and two N-terminal PDZ (postsynaptic-density-95/disc-large/ZO1 homology) domains and is able to form head-to-tail intramolecular conformation to regulate its interactions.

Product Specifications

Product Name Alternative

SLC9A3R1||NHERF||NHERF1

Gene Name

SLC9A3R1

Gene ID

9368

UniProt

O14745

Host

Mouse

Reactivity

Human

Immunogen

Bacterially produced recombinant full-length human NHERF1.

Target Antigen

NHERF1 / EBP50

Clonality

Monoclonal

Clone

EBP-10

Applications

IP, WB, IHC

Purification

Purified by protein-A affinity chromatography

Format

Purified

Storage Conditions

Store at 2-8°C. Do not freeze.

Applications Notes

Immunoprecipitation Western Blotting Recommended dilution: 2 μg/mL, 60 min on vertical incubator Positive control:RAJI human lymphoma cell lysate Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail) . Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. 10% separating SDS-PAGE gel. Immunohistochemistry Recommended dilution: 5 μg/mL